ABSTRACT
【Objective】 To study the expression of leptin mRNA in Chinese obese subjects using dot blot hybridization with a digoxigenin labele d probe. 【Methods】 11 Chinese nonobese subjects (bodymass index, BMI 21.0 kg/ m2±1.5 kg/m2) and 12 Chinese obese subjects(BMI 28.5 kg/m2±2.3 kg/m 2) parti cipated in the study. The human leptin cDNA probe was labeled wih digoxigenin(DI G) b y the random priming method. Expression of leptin mRNA in abdominal adipose tiss ue has been examined using dot blot hybridization with this probe. 【Results】 T he expression of leptin mRNA was significantly higher in obese subjects than in non-obese ones (312.8±108.9 vs 175.9±81.5, relative units, P<0.0 5), an d correlated with BMI (r=0.56, P<0.05). 【Conclusions】 Leptin mRNA le vel is high in obese subjects, and correlated with BMI.
ABSTRACT
Plasma testosterone (T) and estradiol (E2) concentrations were assayed by RIA in 95 patients with NIDDM (48 men and 47 women)and 93 non-diabetic controls (43 men and 50 women), all of them were at or over fifty years of age and all the women were postmenopausal. It was found that in male diabetics, the plasma T level was much lower than that of the controls (P0.05).It is suggested that elevation of E2/T ratio might be a risk factor in the development of CHD in male NIDDM patients, while in female patients the relationship was not clearly demonstrated.